ORIGINAL ARTICLE
Detection of Helicobacter pylori in faeces of children of rural human settlements: An exploratory study in Peru
 
More details
Hide details
1
Graduate School, Universidad Norbert Wiener, Lima, PERU
 
2
Infectious Unit, Nesh Hubbs, Lima, PERU
 
3
Faculties of Health Science, Universidad Tecnológica del Perú, Lima, PERU
 
4
School of Medical Technologist, Faculties of Health Science, Universidad Privada San Juan Bautista, Ica, PERU
 
5
School of Medicine, Faculties of Health Science, Universidad Norbert Wiener, Lima, PERU
 
6
Department of Pathology, Hospital Nacional Docente Madre Niño San Bartolomé, Lima, PERU
 
 
Online publication date: 2022-10-26
 
 
Publication date: 2023-01-01
 
 
Electron J Gen Med 2023;20(1):em424
 
KEYWORDS
ABSTRACT
Introduction:
Helicobacter pylori is one of the major infectious agents in low-income countries, and its clinical characteristics are unknown in many populations. Children are a susceptible population and can contract these pathogens through contaminated food and water.

Objectives:
To determine the presence of Helicobacter pylori in children under eight years of age of rural human settlements in Peru.

Methods:
This exploratory study was conducted with 25 child volunteers authorized by the parents for their evaluation, without precedent of any apparent clinical manifestation. Antigen detection of H. pylori in faeces by a rapid assay onsite H. pylori Ag. Rapid test according to the manufacturer’s requirements. Has been evaluated the social determinants related to possible infection.

Results:
The average age (2±1.8 years) of 14 girls was different from the average age (2.7±2.2 years) of 11 boys included in the study (p=0.010). Of the total we determinate nine (36%) positive tests, which mainly affected boys (five patients 20%) under two years of age (six patients, 24%). In general, ~90% wash their hands with soap and water and ~70% wash fruits and vegetables with water before eating. In addition, >50% ate foods outside of home and ~24% was asymptomatic and did not have a specific meal schedule.

Conclusions:
Among rural children, Helicobacter pylori antibody was detected in 36% of the participants, unaffected by gender, and presenting specific dietary patterns.

REFERENCES (30)
1.
Linz B, Balloux F, Moodley Y, et al. An African origin for the intimate association between humans and helicobacter pylori. Nature. 2007;445:915-8. https://doi.org/10.1038/nature... PMid:17287725 PMCid:PMC1847463.
 
2.
Correa P, Piazuelo MB. Natural history of helicobacter pylori infection. Dig Liver Dis. 2008;40:490-6. https://doi.org/10.1016/j.dld.... PMid:18396115 PMCid:PMC3142999.
 
3.
Hooi KY, Ying LW, Khoon NW, et al. Global prevalence of helicobacter pylori infection: Systematic review and meta-analysis. Gastroenterology. 2017;153:420-9. https://doi.org/10.1053/j.gast... PMid:28456631.
 
4.
Javed S, Skoog EC, Solnick JV. Impact of helicobacter pylori virulence factors on the host immune response and gastric pathology. Curr Top Microbiol Immunol. 2019;421:21-52. https://doi.org/10.1007/978-3-... PMid:31123884.
 
5.
Haley KP, Gaddy JA. Nutrition and helicobacter pylori: Host diet and nutritional immunity influence bacterial virulence and disease outcome. Gastroenterol Res Pract. 2016;2016:3019362. https://doi.org/10.1155/2016/3... PMid:27688750 PMCid:PMC5027306.
 
6.
Nemolato ARM, Moya-Salazar J. The possible role of microbiome in gastric cancer: perspectives. EC Gastroenterology and Digestive System 2018; 5(5): 328-31. Available at: https://www.semanticscholar.or....
 
7.
Holcombe C. Helicobacter pylori: The African enigma. Gut. 1992;33(4):429-31. https://doi.org/10.1136/gut.33... PMid:1582581 PMCid:PMC1374052.
 
8.
Yan X, Li-Ya Z, Hao-Ping L, Jin-Zhe L. Recurrence of Helicobacter pylori infection incidence and influential factors. Chin Med J. 2019;132(7):765-71. https://doi.org/10.1097/CM9.00... PMid:30897591 PMCid:PMC6595863.
 
9.
Ruiz EF, Torres-Roman SJ, Servand SA, et al. Trends and geographic pattern of stomach cancer mortality in Peru. Cancer Epidemiol. 2019;58:193-8. https://doi.org/10.1016/j.cane... PMid:30522779.
 
10.
Curado MP, Oliveira MM, Fagundes M. Prevalence of helicobacter pylori infection in Latin America and the Caribbean populations: A systematic review and meta-analysis. Cancer Epidemiol. 2019;60:141-8. https://doi.org/10.1016/j.cane... PMid:31009922.
 
11.
Mišak Z, Hojsak I, Homan M. Review: Helicobacter pylori in pediatrics. Helicobacter. 2019;24(Suppl. 1):e12639. https://doi.org/10.1111/hel.12... PMid:31486243.
 
12.
Aguilar-Luis MA, Palacios-Cuervo F, Espinal-Reyes F, et al. Highly clarithromycin-resistant helicobacter pylori infection in asymptomatic children from a rural community of Cajamarca-Peru. BMC Res Notes. 2018;11(1):809. https://doi.org/10.1186/s13104... PMid:30428917 PMCid:PMC6236992.
 
13.
World Medical Association. WMA Declaration of Helsinki–Ethical principles for medical research involving human subjects. Fortaleza:64th WMA General Assembly;2013.
 
14.
Magalhães QD, Saito M, Aguiar RG, et al. Helicobacter pylori infection in infants and toddlers in South America: Concordance between [13C] urea breath test and monoclonal H. pylori stool antigen test. J Clin Microbiol. 2013;51(11):3735-40. https://doi.org/10.1128/JCM.01... PMid:24006009 PMCid:PMC3889760.
 
15.
Tseng CH, Tseng FH. Diabetes and gastric cancer: The potential links. World J Gastroenterol. 2014;20(7):1701-11. https://doi.org/10.3748/wjg.v2... PMid:24587649 PMCid:PMC3930970.
 
16.
United Nations. The sustainable development goals report. New York, NY: United Nations Publications, 2016.
 
17.
Cervantes GE. Helicobacter pylori diagnóstico y tratamiento [Helicobacter pylori diagnosis and treatment]. Rev Latinoam Patol Clin Med Lab. 2016;63(4):179-89.
 
18.
Chey W, Wong BC. American College of Gastroenterology guideline on the management of helicobacter pylori infection. Am J Gastroenterol. 2007;102(8):1808-25. https://doi.org/10.1111/j.1572... PMid:17608775.
 
19.
Seo JH, Park JS, Rhee KH, Youn HS. Diagnosis of helicobacter pylori infection in children and adolescents in Korea. Pediatr Gastroenterol Hepatol Nutr. 2018;21(4):219-33. https://doi.org/10.5223/pghn.2... PMid:30345235 PMCid:PMC6182473.
 
20.
Friedt M, Welsch S. An update on pediatric endoscopy. Eur J Med Res. 2013;18(1):24. https://doi.org/10.1186/2047-7... PMid:23885793 PMCid:PMC3751043.
 
21.
World Health Organization. WHO publishes list of bacteria for which new antibiotics are urgently needed. Geneva: WHO; 2017. https://www.who.int/news-room/... (Accessed 16 May 2022).
 
22.
Ruiseco MA, García PW, Davas AY, Hernández RL. Importancia de helicobacter pylori en pediatría, estudio diagnóstico en un grupo de niños [Importance of helicobacter pylori in pediatrics, diagnostic study in a group of children]. Rev Cub Ped. 2017;89(3):290-8. Available at: http://scielo.sld.cu/scielo.ph....
 
23.
Jaime F, Villagrán A, Serrano C, Cerda J, Harris PR. Prevalencia de la infección por helicobacter pylori en niños: Estimando la edad de adquisición [Prevalence of Helicobacter pylori infection in children: Estimating the age of acquisition]. Rev Med Chile. 2013;141:1249-54. https://doi.org/10.4067/S0034-... PMid:24522352.
 
24.
Muñoz UA, Cok GJ, Bussalleu RA, Cetraro CD, Maruy SA, Takami AF. Helicobacter pylori en niños atendidos en el Hospital Nacional Cayetano Heredia durante los años 2003 al 2006 [Helicobacter pylori in children treated at the Hospital Nacional Cayetano Heredia during the years 2003 to 2006]. Rev Gastroenterol Perú. 2008;28(2):109-18. Available at: http://www.scielo.org.pe/sciel....
 
25.
Hulten K, Han SW, Enroth H, et al. Helicobacter pylori in the drinking water in Peru. Gastroenterology. 1996;110(4):1031-5. https://doi.org/10.1053/gast.1... PMid:8612990.
 
26.
Fondo de Cooperación para el Desarrollo Social [Cooperation Fund for Social Development]. Mapa de pobreza provincial y distrital. El enfoque de la pobreza monetaria. 2009 [Provincial and district poverty map. The monetary poverty approach. 2009]. Lima: FONCODES;2010.
 
27.
Lehours P, Ferrero RL. Review: Helicobacter: Inflammation, immunology, and vaccines. Helicobacter. 2019;24(S1): e12644. https://doi.org/10.1111/hel.12... PMid:31486236.
 
28.
Müller A, Oertli M, Arnold IC. H. pylori exploits and manipulates innate and adaptive immune cell signaling pathways to establish persistent infection. Cell Com Sign. 2011;9:25. https://doi.org/10.1186/1478-8... PMid:22044597 PMCid:PMC3214186.
 
29.
Brawner KM, Kumar R, Serrano CA, et al. Helicobacter pylori infection is associated with an altered gastric microbiota in children. Mucosal Immunol. 2017;10:1169-77. https://doi.org/10.1038/mi.201... PMid:28120843 PMCid:PMC5526746.
 
30.
Xiang NQ, Xinhui FN, Loke W, et al. Is there an association between helicobacter pylori infection and irritable bowel syndrome? A meta-analysis. World J Gastroenterol. 2019;25(37):5702-10. https://doi.org/10.3748/wjg.v2... PMid:31602169 PMCid:PMC6785524.
 
eISSN:2516-3507
Journals System - logo
Scroll to top