Aim: Pityriasis Rosea (PR) is an acute inflamatory skin disease with unknown etiology considered to be the most associated with infections. In this study, it was aimed to investigate the role of influenza viruses A-B, parainfluenza viruses 1-3, respiratory syncytial virus (RSV) and adenovirus in the etiology of PR patients. Method: Thirty out-patients, applying to the dermatology department of Konya Education and Research Hospital, who were diagnosed with PR, and healty PR negative control group at the same number, age and sex with PR patients were included into the study. In order to enlighten whether an association of PR with the influenza viruses A-B, parainfluenza viruses 1-3, RSV and adenovirus viruses existed or not. RNA materials from influenza viruses A-B, parainfluenza viruses 1-3 and RSV; DNA material from adenovirus were investigated by using DNA reverse-hybridization method in the nasofarengeal samples obtained from patients with PR and control groups included to the study. Result: No RNA positivity was determined, related to influenza viruses A-B, parainfluenza viruses 1-3, RSV and DNA to adenovirus in any of the patients and control groups. Conclusion: As a consequence, viral etiology of the PR disease still remains unknown, whether to be viral or not, thus further studies are needed to enlighten the unknown etiology of the disease.