Aim: Noise is described as disturbing and unwanted sound. In this study, we aimed to investigate the effect of noise on oxidative stress parameters in rat. Methods: Twenty male Sprague-Dawley rats were used in the study. Noise group (n=10) was exposed to noise for 20 days / 4 hour 100 dB. Control group (n=10) that was not exposed to any noise and was kept from any stress source, was hold in the same conditions. Baseline and after 20th day of the experiment, blood samples of rats were collected and their sera were separated. Malondialdehyde (MDA), nitric oxide (NO) levels and glutathione peroxidase (GSH-Px) activity were analyzed in rat sera. Results: MDA and NO levels and GSH-Px activities were found to be increased significantly at the end of experiment in the group exposed to noise. No parameters were significantly differed between at baseline and at the end of experiment in the control group. Conclusion: The present study showing an elevation in MDA level, an indicator of lipid peroxidation, as well as NO level and GSH-Px activity by noise exposure suggests the presence of oxidative stress which may lead to various degrees of damages in the cells, mainly via lipid peroxidation pathway.in the noise group. Therefore, these results appear to support the fact that noise might cause damage not only in the ears but also in whole body leading to oxidative stress.