Chancroid is a sexually transmitted infection caused by the Gram-negative bacteria Haemophilus ducreyi. The disease manifests as genital ulcerations, which may be accompanied by lymphoadenitis. It is widespread in low-resource countries in Africa and can co-occur with human immunodeficiency virus. We present a case of chancroid in patient with Candida glabrata vaginitis. There were two major genital ulcers, one of which was a painful ulcer in the left labia major and the other was a painless ulcer in the cervix uteri; she was also found upon examination to have bilateral inguinal lymphadenopathy. Candida glabrata was found in vaginal culture. We treated the patient with azithromycin 1 gram orally for 3 days, and fluconazole 150 mg oral and vaginally. This case is very typical of an H. ducreyi infection, and physicians should be aware of this type of infection when carrying out routine gynecological examinations.